UGP2 antibody (Biotin)

Catalog No. ABIN568503

The Biotin-conjugated Rabbit Polyclonal anti-UGP2 antibody (ABIN568503) specifically detects UGP2 in WB, IF, DB, EIA, ID, IP and RIA. The antibody is reactive with Cow samples.

Quick Overview for UGP2 antibody (Biotin) (ABIN568503)

Target: UGP2 (UDP-Glucose Pyrophosphorylase 2 (UGP2))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UGP2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Product Details anti-UGP2 Antibody (Biotin)

Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~5.0

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Uridine-5’-diphosphoglucose Pyrophosphorylase isolated and purified from Bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for UGP2

Target: UGP2 (UDP-Glucose Pyrophosphorylase 2 (UGP2))

Alternative Name: UGP2

Background: UGP2 is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue,UDP-glucose is a direct precursor of glycogen, in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. The eukaryotic enzyme has no significant sequence similarity to the prokaryotic enzyme.Synonyms: UDP-glucose pyrophosphorylase, UDPGP, UGP1, UGPase, UTP-glucose-1-phosphate uridylyltransferase

Gene ID: 281565

NCBI Accession: NP_776637

UniProt: Q07130

Pathways: Cellular Glucan Metabolic Process