Lactoferrin antibody

Catalog No. ABIN568585

This Goat Polyclonal antibody specifically detects Lactoferrin in WB, IP, IF, EIA, ID and DB. It exhibits reactivity toward Human.

Quick Overview for Lactoferrin antibody (ABIN568585)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Lactoferrin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB)

Product Details anti-Lactoferrin Antibody

Specificity: In immunoelectrophoresis against human milk a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma. Cross-reactivity:The antiserum does not cross-react with any other human plasma proteins as tested in geldiffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction with Rhesus monkey milk has been observed.

Purification: Fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography

Immunogen: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from human milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1,0 mL sterile distilled water

Concentration: 10 mg/mL

Buffer: Phosphate buffered saline, pH 7.2 - No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactotransferrin

Background: Synonyms: EC=3.4.21, LF, LTF, Lactoferrin, Talalactoferrin

Gene ID: 4057

NCBI Accession: NP_002334

UniProt: P02788

Pathways: Transition Metal Ion Homeostasis