FGF10 antibody (Biotin)

Catalog No. ABIN5687582

The Biotin-conjugated Goat Polyclonal anti-FGF10 antibody (ABIN5687582) specifically detects FGF10 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for FGF10 antibody (Biotin) (ABIN5687582)

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FGF10 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-FGF10 Antibody (Biotin)

Purification: Anti-hFGF-10 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFGF-10 (human FGF-10).

Application Details

Application Notes: ELISA:

To detect hFGF-10 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hFGF-10. Sandwich: To detect hFGF-10 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FGF-10 (XP-5131) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-10.

Western Blot:
To detect hFGF-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: FGF-10 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for FGF10

Target: FGF10 (Fibroblast Growth Factor 10 (FGF10))

Alternative Name: FGF-10

Gene ID: 2255

UniProt: O15520

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Tube Formation, Positive Regulation of Response to DNA Damage Stimulus