IL-2 antibody (Biotin)

Catalog No. ABIN5687588

This anti-IL-2 antibody is a Goat Polyclonal antibody detecting IL-2 in WB and ELISA. Suitable for Human.

Quick Overview for IL-2 antibody (Biotin) (ABIN5687588)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-2 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL-2 Antibody (Biotin)

Purification: Anti-hIL-2 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL-2 (human IL-2).

Application Details

Application Notes: ELISA:

To detect hIL-2 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-2.

Sandwich:

To detect hIL-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-2 (XP-5182) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-2.

Western Blot:

To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background: Interleukin 2 (IL2) is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. IL2 has been shown to have antitumor effects in some studies. This is probably mediated by cytotoxic effector cells.

Gene ID: 3558

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation