Neurotrophin 4 antibody (Biotin)

Catalog No. ABIN5687603

This anti-Neurotrophin 4 antibody is a Goat Polyclonal antibody detecting Neurotrophin 4 in WB and ELISA. Suitable for Human.

Quick Overview for Neurotrophin 4 antibody (Biotin) (ABIN5687603)

Target: Neurotrophin 4 (NTF4)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Neurotrophin 4 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Neurotrophin 4 Antibody (Biotin)

Purification: Anti-hNT-4 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNT-4 (human NT-4).

Application Details

Application Notes: ELISA:

To detect hNT-4 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hNT-4.

Sandwich:

To detect hNT-4 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human NT-4 (XP-5251) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-4.

Western Blot:

To detect hNT-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: NT-4 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Neurotrophin 4

Target: Neurotrophin 4 (NTF4)

Alternative Name: NT-4

Gene ID: 4909

UniProt: P34130

Pathways: RTK Signaling