KIT Ligand antibody (Biotin)

Catalog No. ABIN5687609

This anti-KIT Ligand antibody is a Goat Polyclonal antibody detecting KIT Ligand in WB and ELISA. Suitable for Human.

Quick Overview for KIT Ligand antibody (Biotin) (ABIN5687609)

Target: KIT Ligand (KITLG)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-KIT Ligand Antibody (Biotin)

Purification: Anti-hSCF specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hSCF (human Stem Cell Factor).

Application Details

Application Notes: ELISA:

To detect hSCF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.

Sandwich:

To detect hSCF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human SCF (XP-5265) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hSCF.

Western Blot:

To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: SCF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: SCF

Background: Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

Gene ID: 4254

UniProt: P21583

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway