Thrombopoietin antibody (Biotin)

Catalog No. ABIN5687618

The Biotin-conjugated Goat Polyclonal anti-Thrombopoietin antibody (ABIN5687618) specifically detects Thrombopoietin in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for Thrombopoietin antibody (Biotin) (ABIN5687618)

Target: Thrombopoietin (THPO)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Thrombopoietin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Thrombopoietin Antibody (Biotin)

Purification: Anti-hTPO specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO (human TPO).

Application Details

Application Notes: ELISA:

To detect hTPO by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hTPO.

Sandwich:

To detect hTPO by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TPO (XP-5288) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.

Western Blot:
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: TPO antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Thrombopoietin

Target: Thrombopoietin (THPO)

Alternative Name: Thrombopoietin (TPO)

Background: Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Gene ID: 7066

UniProt: P40225

Pathways: JAK-STAT Signaling, Hormone Activity