CCL24 antibody (Biotin)

Catalog No. ABIN5687627

The Biotin-conjugated Goat Polyclonal anti-CCL24 antibody (ABIN5687627) specifically detects CCL24 in WB and ELISA. The antibody is reactive with Mouse samples.

Quick Overview for CCL24 antibody (Biotin) (ABIN5687627)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL24 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CCL24 Antibody (Biotin)

Purification: Anti-mEotaxin-2 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mEotaxin-2 (murine Eotaxin-2).

Application Details

Application Notes: ELISA:

To detect mEotaxin-2 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mEotaxin-2.

Sandwich: To detect mEotaxin-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine Eotaxin-2 (XP-5125) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin-2.

Western Blot:
To detect mEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Eotaxin-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL24

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Alternative Name: Eotaxin-2

Background: Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.

Gene ID: 56221

UniProt: Q9JKC0

Pathways: Regulation of Actin Filament Polymerization