M-CSF/CSF1 antibody

Catalog No. ABIN5687632

The Goat Polyclonal anti-M-CSF/CSF1 antibody has been validated for WB, ELISA and Neut. It is suitable to detect M-CSF/CSF1 in samples from Mouse.

Quick Overview for M-CSF/CSF1 antibody (ABIN5687632)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This M-CSF/CSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-M-CSF/CSF1 Antibody

Purification: Anti-mM-CSF specific antibody was purified by affinity chromatography employing immobilized mM-CSF matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mM-CSF (Murine M-CSF).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mM-CSF (1.5 ng/mL), a concentration of 0.05 - 0.08 μ,g/mL of this antibody is required.

ELISA:

To detect mM-CSF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mM-CSF.

Sandwich:

To detect mM-CSF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine M-CSF (XP-5228Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mM-CSF.

Western Blot:
To detect mM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: M-CSF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for M-CSF/CSF1

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: M-CSF

Gene ID: 12977

UniProt: P07141

Pathways: RTK Signaling