CCL7 antibody (Biotin)

Catalog No. ABIN5687633

This anti-CCL7 antibody is a Goat Polyclonal antibody detecting CCL7 in WB and ELISA. Suitable for Mouse.

Quick Overview for CCL7 antibody (Biotin) (ABIN5687633)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL7 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-CCL7 Antibody (Biotin)

Purification: Anti-mMCP-3 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mMCP-3 (murine MCP-3).

Application Details

Application Notes: ELISA:

To detect mMCP-3 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMCP-3.

Sandwich:

To detect mMCP-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine MCP-3 (XP-5223) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.

Western Blot:

To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MCP-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: MCP-3

Background: This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.

Gene ID: 20306

UniProt: Q03366