IL1A antibody (Biotin)

Catalog No. ABIN5687645

This anti-IL1A antibody is a Goat Polyclonal antibody detecting IL1A in WB and ELISA. Suitable for Rat.

Quick Overview for IL1A antibody (Biotin) (ABIN5687645)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IL1A antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL1A Antibody (Biotin)

Purification: Anti-Rat IL-1-α, specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-1-α, (Rat IL-1-α,).

Application Details

Application Notes: ELISA:

To detect Rat IL-1-α, by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant Rat IL-1-α,.

Sandwich:

To detect Rat IL-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat IL-1α, (XP-5176) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1α,.

Western Blot:

To detect Rat IL-1-α, by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1-α, is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1a

Gene ID: 24493

UniProt: P16598

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints