Cytokeratin 18 antibody (Internal Region)

Catalog No. ABIN5687958

This Goat Polyclonal antibody specifically detects Cytokeratin 18 in WB and ELISA. It exhibits reactivity toward Human and Pig.

Quick Overview for Cytokeratin 18 antibody (Internal Region) (ABIN5687958)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Cytokeratin 18 Antibody

Binding Specificity: Internal Region

Specificity: Reported variants represent identical protein: NP_000215.1, NP_954657.1.

Purification: keratin 18 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: keratin 18 Antibody was raised against synthetic peptide EELDKYWSQQIE found at an internal region of keratin 18.

Application Details

Application Notes: ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 48  kDa band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1  kDa according to NP_000215.1). Recommended concentration: 0.1-0.3μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: keratin 18 is supplied in Tris saline, 0.02 % sodium azide,  pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Minimize freezing and thawing.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: keratin 18

Molecular Weight: Approx 48kDa

Gene ID: 3875

NCBI Accession: NP_000215

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis