IL-10 antibody

Catalog No. ABIN5688698

This anti-IL-10 antibody is a Mouse Monoclonal antibody detecting IL-10 in WB and ELISA. Suitable for Rat.

Quick Overview for IL-10 antibody (ABIN5688698)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2F4-11-1

Product Details anti-IL-10 Antibody

Purification: This antibody was purified from tissue culture by Protein A affinity chromatography.

Immunogen: E.coli derived Recombinant Rat IL-10

Application Details

Application Notes: ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μ,L/well), a concentration of 8.0-16.0 μ,g/mL of this antibody will detect at least 500 pg/mL of recombinant Rat IL-10 when used with ProSci's Polyclonal Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (XP-5163Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western Blot:

To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μ,g/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10

Gene ID: 3586

NCBI Accession: NP_000563

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints