CCL3 antibody

Catalog No. ABIN5688699

This Mouse Monoclonal antibody specifically detects CCL3 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for CCL3 antibody (ABIN5688699)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: 1-2_3E8-2H6-2B6

Product Details anti-CCL3 Antibody

Purification: This IgG1 antibody was purified from ascites fluid by SAS precipitation followed by ion exchange column.

Immunogen: The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMIP-1α, as the immunizing antigen.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1α, (100 ng/mL), a concentration of 5.0-10.0 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1α, when used with our biotinylated antigen affinity purified anti-human MIP-1α, (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μ,g/mL.

Western Blot:

To detect hMIP-1α, by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1α, is 1.0-2.0 ng/lane, under non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: MIP-1α, lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: MIP-1a

Background: Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

Gene ID: 6348

UniProt: P10147

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy