CCL7 antibody

Catalog No. ABIN5688702

This Mouse Monoclonal antibody specifically detects CCL7 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for CCL7 antibody (ABIN5688702)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: PEPY042_hMCP3

Product Details anti-CCL7 Antibody

Purification: This IgG1 antibody was purified from ascites fluid on a Protein A affinity column.

Immunogen: The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMCP-3 as the immunizing antigen.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-3 (100 ng/mL), a concentration of 1 - 3 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect recombinant human MCP-3 when used with our biotinylated antigen affinity purified anti-human MCP-3 (XP-5221Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western Blot:

To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: MCP-3 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: MCP-3

Background: MCP-3 is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.

Gene ID: 6354

UniProt: P80098