Leptin antibody

Catalog No. ABIN5688720

This Mouse Monoclonal antibody specifically detects Leptin in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for Leptin antibody (ABIN5688720)

Target: Leptin (LEP)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: G1_4B6-3

Product Details anti-Leptin Antibody

Purification: This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

Immunogen: Leptin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Leptin as the immunizing antigen.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μ,g/mL of this antibody is required. ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 4.0-8.0 μ,g/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with our biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μ,g/mL.

Western Blot:

To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: Leptin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.

Was product number XP-5712-M

Gene ID: 3952

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process