IL-10 antibody

Catalog No. ABIN5688729

This Mouse Monoclonal antibody specifically detects IL-10 in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for IL-10 antibody (ABIN5688729)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: 15B9-1-2

Product Details anti-IL-10 Antibody

Purification: This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.

Immunogen: E.coli derived Recombinant Murine IL-10

Application Details

Application Notes: ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μ,L/well), a concentration of 4.0-8.0 μ,g/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with ProSci's Polyclonal biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western Blot:

To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μ,g/mL of this antibody is required.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10

Gene ID: 3586

NCBI Accession: NP_000563

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints