Fibrillarin antibody

Catalog No. ABIN5688751

The Mouse Monoclonal anti-Fibrillarin antibody has been validated for WB and IHC. It is suitable to detect Fibrillarin in samples from Human, Rat and Drosophila melanogaster.

Quick Overview for Fibrillarin antibody (ABIN5688751)

Target: Fibrillarin (FBL)

Reactivity: Human, Rat, Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibrillarin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 38F3

Product Details anti-Fibrillarin Antibody

Specificity: Specific for the ~34  kDa Fibrillarin /Nop1p protein.

Purification: Total IgG fraction

Immunogen: Yeast nuclear preparations.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: -20 °C

Storage Comment: Fibrillarin antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

Target Details for Fibrillarin

Target: Fibrillarin (FBL)

Alternative Name: Fibrillarin

Background: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Molecular Weight: 34 kDa

Gene ID: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process