GABRA1 antibody (Cytoplasmic Loop)

Catalog No. ABIN5688874

The Rabbit Polyclonal anti-GABRA1 antibody is suitable to detect GABRA1 in samples from Rat, Human, Mouse, Cow, Dog, Chicken, Zebrafish (Danio rerio) and Xenopus laevis. It has been validated for WB.

Quick Overview for GABRA1 antibody (Cytoplasmic Loop) (ABIN5688874)

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reactivity: Rat, Human, Mouse, Cow, Dog, Chicken, Zebrafish (Danio rerio), Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GABRA1 Antibody

Binding Specificity: Cytoplasmic Loop

Purification: Affinity Purified

Immunogen: Fusion protein from the cytoplasmic loop of the b1-subunit of rat GABAA receptor.

Application Details

Application Notes: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate, Xenopus, and zebra fish based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: GABAA Receptor antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

Target Details for GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Alternative Name: GABAA Receptor

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003).

Molecular Weight: 55 kDa

Gene ID: 25450

UniProt: P15431