Tryptophan Hydroxylase 1 antibody (pSer19)

Catalog No. ABIN5688890

This anti-Tryptophan Hydroxylase 1 antibody is a Rabbit Polyclonal antibody detecting Tryptophan Hydroxylase 1 in WB. Suitable for Mouse, Rat, Cow and Xenopus laevis.

Quick Overview for Tryptophan Hydroxylase 1 antibody (pSer19) (ABIN5688890)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reactivity: Mouse, Rat, Cow, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Tryptophan Hydroxylase 1 Antibody

Binding Specificity: pSer19

Purification: Affinity Purified

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser19 of rat tryptophan hydroxylase 2 (TPH2).

Application Details

Application Notes: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, mouse and zebra fish based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Tryptophan Hydroxylase antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

Target Details for Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Alternative Name: Tryptophan Hydroxylase

Background: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et

Molecular Weight: 55 kDa

Gene ID: 317675

UniProt: Q8CGU9

Pathways: Feeding Behaviour