Connexin 43/GJA1 antibody (pSer368)

Catalog No. ABIN5688898

The Rabbit Polyclonal anti-Connexin 43/GJA1 antibody has been validated for WB. It is suitable to detect Connexin 43/GJA1 in samples from Human, Rat, Mouse, Dog, Cow, Chicken, Zebrafish (Danio rerio) and Goat.

Quick Overview for Connexin 43/GJA1 antibody (pSer368) (ABIN5688898)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Chicken, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: pSer368

Purification: Affinity Purified

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser368 of rat connexin 43.

Application Details

Application Notes: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, guinea pig, human, mouse and zebra fish based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Connexin 43 antibody can be stored at -20°C and is stable at -20°C for at least 1 year.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Connexin 43

Background: Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin 43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin 43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002, Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin 43 that results in a decrease in gap junction permeability (Bao et al., 2004b).

Molecular Weight: 43 kDa

Gene ID: 24392

UniProt: P08050

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization