Tyrosine Hydroxylase antibody (pSer40)

Catalog No. ABIN5688900

This Rabbit Polyclonal antibody specifically detects Tyrosine Hydroxylase in WB, IHC and IF. It exhibits reactivity toward Human, Mouse, Pig and Monkey.

Quick Overview for Tyrosine Hydroxylase antibody (pSer40) (ABIN5688900)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Human, Mouse, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Tyrosine Hydroxylase Antibody

Binding Specificity: pSer40

Specificity: Tyrosine Hydroxylase antibody is specific for the 60k rat TH phosphorylated at Ser40 in lysates of PC-12 Cells stimulated by Okadaic Acid (1 μM for 60 minutes). Some higher molecular weight bands may be detected depending upon the brain region being studied, protein loads, and the detection methods used. See cited reference.

Purification: Affinity Purified

Immunogen: TH (Ser40) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase (TH).

Application Details

Application Notes: Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Human, porcine, quail, mouse, and non-human primate have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase

Background: Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons. We raised this polyclonal antibody against a peptide representing the sequence around Ser40 in rat TH. This antibody is suitable for most immunochemical applications in a variety of mammalian and some non-mammalian species.

Molecular Weight: 60 kDa

Gene ID: 25085

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour