Glutamate Receptor 1 antibody (pSer845)

Catalog No. ABIN5688909

This Rabbit Polyclonal antibody specifically detects Glutamate Receptor 1 in WB, IHC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Glutamate Receptor 1 antibody (pSer845) (ABIN5688909)

Target: Glutamate Receptor 1 (GLUR1)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutamate Receptor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Glutamate Receptor 1 Antibody

Binding Specificity: pSer845

Specificity: GluR1 antibody is specific for GluR1 phosphorylated at Ser845.

Purification: Affinity Purified

Immunogen: GluR1 (Ser845) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser845 of rat GluR1.

Application Details

Application Notes: Immunolabeling of the GluR1 protein in western blots of rat brain is blocked by the Ser845 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Target Details for Glutamate Receptor 1

Target: Glutamate Receptor 1 (GLUR1)

Alternative Name: GluR1

Background: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of 4 distinct subunits (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser845. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.

Molecular Weight: 100 kDa

Gene ID: 50592

UniProt: P19490

Pathways: PI3K-Akt Signaling