PRAS40 antibody (AA 190-240)

Catalog No. ABIN5688960

This Rabbit Polyclonal antibody specifically detects PRAS40 in WB, ELISA, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PRAS40 antibody (AA 190-240) (ABIN5688960)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-PRAS40 Antibody

Binding Specificity: AA 190-240

Specificity: AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist.

Purification: AKT1S1 Antibody is affinity chromatography purified via peptide column.

Immunogen: AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1.

The immunogen is located within amino acids 190 - 240 of AKT1S1.

Isotype: IgG

Application Details

Application Notes: AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: AKT1S1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: AKT1S1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: AKT1S1

Background: AKT1S1 Antibody: The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1). AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2). Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3).

Molecular Weight: Predicted: 30 kDa

Observed: 28 kDa

Gene ID: 84335

NCBI Accession: NP_115751

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect