Cardiotrophin 1 antibody

Catalog No. ABIN5688976

The Rabbit Polyclonal anti-Cardiotrophin 1 antibody has been validated for WB, ELISA and Neut. It is suitable to detect Cardiotrophin 1 in samples from Human.

Quick Overview for Cardiotrophin 1 antibody (ABIN5688976)

Target: Cardiotrophin 1 (CTF1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cardiotrophin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Cardiotrophin 1 Antibody

Purification: Anti-hCardiotrophin 1 specific antibody was purified by affinity chromatography employing immobilized hCardiotrophin 1 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCardiotrophin 1 (human Cardiotrophin-1).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hCardiotrophin 1 (15 ng/mL), a concentration of 0.25 - 0.40 μ,g/mL of this antibody is required.

ELISA:

To detect hCardiotrophin 1 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hCardiotrophin 1.

Western Blot:
To detect hCardiotrophin 1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCardiotrophin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Cardiotrophin-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Cardiotrophin 1

Target: Cardiotrophin 1 (CTF1)

Alternative Name: Cardiotrophin-1

Gene ID: 1489

UniProt: Q16619

Pathways: JAK-STAT Signaling