IL-25 antibody (Biotin)

Catalog No. ABIN5689037

This anti-IL-25 antibody is a Rabbit Polyclonal antibody detecting IL-25 in WB and ELISA. Suitable for Human.

Quick Overview for IL-25 antibody (Biotin) (ABIN5689037)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-25 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL-25 Antibody (Biotin)

Purification: Anti-hIL-17E specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17E (Human IL-17E).

Application Details

Application Notes: ELISA:

To detect hIL-17E by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-17E.

Sandwich:

To detect hIL-17E by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-17E (XP-5202) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-17E.

Western Blot:

To detect hIL-17E by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17E is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-17E antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-25

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL-17E

Gene ID: 64806

UniProt: Q9H293