FGF7 antibody (Biotin)

Catalog No. ABIN5689056

This Rabbit Polyclonal antibody specifically detects FGF7 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for FGF7 antibody (Biotin) (ABIN5689056)

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF7 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-FGF7 Antibody (Biotin)

Purification: Anti-hKGF specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hKGF (human Keratinocyte Growth Factor).

Application Details

Application Notes: ELISA:

To detect hKGF by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hKGF.

Sandwich:

To detect hKGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human KGF (XP-5208) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hKGF.

Western Blot:
To detect hKGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: KGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for FGF7

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Alternative Name: KGF

Background: KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells.

Gene ID: 2252

UniProt: P21781

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway