XCL1 antibody

Catalog No. ABIN5689063

The Rabbit Polyclonal anti-XCL1 antibody is suitable to detect XCL1 in samples from Human. It has been validated for WB, ELISA and Neut.

Quick Overview for XCL1 antibody (ABIN5689063)

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XCL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-XCL1 Antibody

Purification: Anti-hLymphotactin specific antibody was purified by affinity chromatography employing immobilized hLymphotactin matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLymphotactin (human Lymphotactin).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hLymphotactin (60 ng/mL), a concentration of 1.7 - 3.0 μ,g/mL of this antibody is required.

ELISA:

To detect hLymphotactin by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hLymphotactin.

Sandwich:

To detect hLymphotactin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Lymphotactin (XP-5217Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLymphotactin.

Western Blot:

To detect hLymphotactin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLymphotactin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Lymphotactin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for XCL1

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Alternative Name: Lymphotactin

Gene ID: 6375

UniProt: P47992

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation