CCL17 antibody

Catalog No. ABIN5689115

This anti-CCL17 antibody is a Rabbit Polyclonal antibody detecting CCL17 in WB, ELISA and Neut. Suitable for Human.

Quick Overview for CCL17 antibody (ABIN5689115)

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL17 Antibody

Purification: Anti-hTARC specific antibody was purified by affinity chromatography employing immobilized hTARC matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTARC (human Thymus and Activation Regulated Chemokine).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTARC (100.00 ng/mL), a concentration of 20.0 - 30 μ,g/mL of this antibody is required.

ELISA:

To detect hTARC by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTARC.

Sandwich:

To detect hTARC by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TARC (XP-5279Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTARC.

Western Blot:
To detect hTARC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTARC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Tarc antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL17

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Alternative Name: Tarc

Background: Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TARC displays chemotactic activity for T lymphocytes, but not monocytes or granulocytes. TARC binds to chemokine receptors CCR4 and CCR8. This chemokine plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells

Gene ID: 6361

UniProt: Q92583