TGFA antibody

Catalog No. ABIN5689119

This Rabbit Polyclonal antibody specifically detects TGFA in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for TGFA antibody (ABIN5689119)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-TGFA Antibody

Purification: Anti-hTGF-alpha specific antibody was purified by affinity chromatography employing immobilized hTGF-alpha matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTGF-alpha (human Transforming Growth Factor-alpha).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTGF-alpha (2 ng/mL), a concentration of 0.1 - 0.15 μ,g/mL of this antibody is required.

ELISA:

To detect hTGF-alpha by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTGF-alpha.

Sandwich:

To detect hTGF-α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TGF-α, (XP-5281Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-α,.

Western Blot:
To detect hTGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: TGF-a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF-a

Gene ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway