FGF9 antibody

Catalog No. ABIN5689132

The Rabbit Polyclonal anti-FGF9 antibody is suitable to detect FGF9 in samples from Mouse. It has been validated for WB, ELISA and Neut.

Quick Overview for FGF9 antibody (ABIN5689132)

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-FGF9 Antibody

Purification: Anti-mFGF-9 specific antibody was purified by affinity chromatography employing immobilized mFGF-9 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mFGF-9 (murine FGF-9).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mFGF-9 (1.50 ng/mL), a concentration of 0.025 - 0.06 μ,g/mL of this antibody is required.

ELISA:

To detect mFGF-9 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mFGF-9.

Sandwich: To detect mFGF-9 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine FGF-9 (XP-5135Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mFGF-9.

Western Blot:

To detect mFGF-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mFGF-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: FGF-9 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for FGF9

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Alternative Name: FGF-9

Gene ID: 14180

UniProt: P54130