G-CSF antibody

Catalog No. ABIN5689134

This Rabbit Polyclonal antibody specifically detects G-CSF in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for G-CSF antibody (ABIN5689134)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G-CSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-G-CSF Antibody

Purification: Anti-mG-CSF specific antibody was purified by affinity chromatography employing immobilized mG-CSF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mG-CSF.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mG-CSF (0.5 ng/mL), a concentration of 0.005 - 0.010 μ,g/mL of this antibody is required.

ELISA:

To detect mG-CSF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mG-CSF.

Sandwich: To detect mG-CSF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine G-CSF (XP-5142Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mG-CSF.

Western Blot:

To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: G-CSF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: G-CSF

Gene ID: 12985

UniProt: P09920

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization