IL-13 antibody

Catalog No. ABIN5689144

The Rabbit Polyclonal anti-IL-13 antibody has been validated for WB, ELISA and Neut. It is suitable to detect IL-13 in samples from Mouse.

Quick Overview for IL-13 antibody (ABIN5689144)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-13 Antibody

Purification: Anti-mIL-13 specific antibody was purified by affinity chromatography employing immobilized mIL-13 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-13 (murine Interleukin-13).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-13 (10 ng/mL), a concentration of 0.08 - 0.10 μ,g/mL of this antibody is required.

ELISA:

To detect mIL-13 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-13.

Sandwich

To detect mIL-13 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-13 (XP-5168Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-13.

Western Blot:
To detect mIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-13 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL-13

Background: L13 inhibits proinflammatory cytokine production and stimulates antibody production. It induces proliferation in the human pre myeloid cell line TF1. IL13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression. IL13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity. Human and mouse interleukin 13 share approximately 58 % amino acid sequence identity. Although human and mouse IL13 are equally active on human cells, human IL13 is much less active than mouse IL13 on mouse cells. Human IL13 and human IL4 also share approximately 30 % sequence homology and have similar biological functions.

Gene ID: 16163

UniProt: P20109

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport