GABRA3 antibody (AA 33-37)

Catalog No. ABIN5689208

The Rabbit Polyclonal anti-GABRA3 antibody (ABIN5689208) specifically detects GABRA3 in WB. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for GABRA3 antibody (AA 33-37) (ABIN5689208)

Target: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GABRA3 Antibody

Binding Specificity: AA 33-37

Specificity: This antibody detects endogenous level of total GABA A Receptor a3 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide

Immunogen: GABA A Receptor a3 antibody was raised against a peptide sequence around aa. 33~37 (R-R-Q-E-P) derived from Rat GABA A Receptor a3.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for GABRA3

Target: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Alternative Name: GABA A Receptor a3

Background: GABA (γ,-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).

Molecular Weight: 51 kDa

Gene ID: 24947

NCBI Accession: NP_058765

UniProt: P20236