CDC25B antibody (pSer149)

Catalog No. ABIN5689217

This anti-CDC25B antibody is a Rabbit Polyclonal antibody detecting CDC25B in WB. Suitable for Mouse and Rat.

Quick Overview for CDC25B antibody (pSer149) (ABIN5689217)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CDC25B Antibody

Binding Specificity: pSer149

Specificity: This antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Cdc25B (Phospho-Ser149) antibody was raised against a peptide sequence around phosphorylation site of Serine 149 (F-R-S (p) -L-P) derived from Mouse Cdc25B.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: Cdc25B

Background: Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Molecular Weight: 62 kDa

Gene ID: 12531

NCBI Accession: NP_001104545

UniProt: Q9DBN8

Pathways: Cell Division Cycle, M Phase, Autophagy