PKM1/2 (AA 36-40) antibody

Catalog No. ABIN5689255

Product Details

Target: PKM1/2

Binding Specificity: AA 36-40

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of total PKM1/2 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: PKM1/2 antibody was raised against a peptide sequence around aa. 36~40 (D-S-P-P-I) derived from Human PKM1/2.

Application Details

Application Notes: Western Blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details

Target: PKM1/2

Alternative Name: PKM1 / 2

Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Molecular Weight: 60 kDa

Gene ID: 5315

NCBI Accession: NP_872270

UniProt: P14618