E2F1 antibody (AA 430-434)

Catalog No. ABIN5689264

The Rabbit Polyclonal anti-E2F1 antibody has been validated for WB. It is suitable to detect E2F1 in samples from Human and Mouse.

Quick Overview for E2F1 antibody (AA 430-434) (ABIN5689264)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-E2F1 Antibody

Binding Specificity: AA 430-434

Specificity: This antibody detects endogenous levels of total E2F-1 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: E2F-1 antibody  was raised against a peptide sequence around aa.430~434 (G-D-L-T-P) derived from Human E2F-1.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F 1 

Background: Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.

Molecular Weight: 70 kDa

Gene ID: 1869

NCBI Accession: NP_005216

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy