EWSR1 antibody (AA 213-217)

Catalog No. ABIN5689269

This Rabbit Polyclonal antibody specifically detects EWSR1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EWSR1 antibody (AA 213-217) (ABIN5689269)

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EWSR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EWSR1 Antibody

Binding Specificity: AA 213-217

Specificity: This antibody detects endogenous levels of total EWS protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: EWS antibody was raised against a peptide sequence around aa.213~217 (T-Y-G-Q-P) derived from Human EWS.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for EWSR1

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Alternative Name: EWS

Background: This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11,22)(q24,q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.

Molecular Weight: 85 kDa

Gene ID: 2130

NCBI Accession: NP_053733

UniProt: Q9BZD1