TWIST1 antibody (AA 18-22)

Catalog No. ABIN5689354

The Rabbit Polyclonal anti-TWIST1 antibody has been validated for WB. It is suitable to detect TWIST1 in samples from Human, Mouse and Rat.

Quick Overview for TWIST1 antibody (AA 18-22) (ABIN5689354)

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWIST1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TWIST1 Antibody

Binding Specificity: AA 18-22

Specificity: This antibody detects endogenous levels of total Twist1 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Twist1 antibody was raised against a peptide sequence around aa.18~22 (S-N-S-E-E) derived from Human Twist1.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: Twist1

Background: Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

Molecular Weight: 28 kDa

Gene ID: 7291

NCBI Accession: NP_000465

UniProt: Q15672

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity