SPARC antibody (AA 52-56)

Catalog No. ABIN5689585

The Rabbit Polyclonal anti-SPARC antibody is suitable to detect SPARC in samples from Human. It has been validated for WB.

Quick Overview for SPARC antibody (AA 52-56) (ABIN5689585)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPARC Antibody

Binding Specificity: AA 52-56

Specificity: This antibody detects endogenous level of total SPARC protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: SPARC antibody was raised against a peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC

Background: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites, an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Molecular Weight: 42 kDa

Gene ID: 6678

NCBI Accession: NP_003109

UniProt: P09486

Pathways: Autophagy