SMC1A antibody (AA 955-959)

Catalog No. ABIN5689614

This Rabbit Polyclonal antibody specifically detects SMC1A in WB and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for SMC1A antibody (AA 955-959) (ABIN5689614)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SMC1A Antibody

Binding Specificity: AA 955-959

Specificity: This antibody detects endogenous level of total SMC1 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: SMC1 (Ab-957) antibody was raised against a peptide sequence around aa. 955~959 (G-S-S-Q-G) derived from Human SMC1.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1

Background: Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Molecular Weight: 145 kDa

Gene ID: 8243

NCBI Accession: NP_006297

UniProt: Q14683

Pathways: Stem Cell Maintenance