BCR antibody (pTyr177)

Catalog No. ABIN5689837

The Rabbit Polyclonal anti-BCR antibody is suitable to detect BCR in samples from Human and Mouse. It has been validated for WB and IHC.

Quick Overview for BCR antibody (pTyr177) (ABIN5689837)

Target: BCR (Breakpoint Cluster Region (BCR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BCR Antibody

Binding Specificity: pTyr177

Specificity: This antibody detects endogenous level of Bcr only when phosphorylated at tyrosine 177.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: Bcr (Phospho-Tyr177) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 177 (P-F-Y (p) -V-N) derived from Human Bcr.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for BCR

Target: BCR (Breakpoint Cluster Region (BCR))

Alternative Name: Bcr

Background: GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.

Molecular Weight: 210 kDa

Gene ID: 613

NCBI Accession: NP_004318

UniProt: P11274

Pathways: Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling