IL-33 antibody

Catalog No. ABIN5690010

This Rabbit Polyclonal antibody specifically detects IL-33 in WB, ELISA, IHC and Neut. It exhibits reactivity toward Human.

Quick Overview for IL-33 antibody (ABIN5690010)

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-33 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut)

Product Details anti-IL-33 Antibody

Purification: IL-33 specific antibody was purified by affinity chromatography employing immobilized hIL-33 matrix.

Immunogen: E.coli derived Recombinant Human IL-33

Application Details

Application Notes: ELISA:
Sandwich:
To detect hIL-33 by sandwich ELISA (using 100μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Polyclonal Biotinylated Anti-Human IL-33 (38-193) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-33.

Western Blot:

To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/mL), a concentration of 0.2-0.4 μ,g/mL of this antibody is required.

Immunohistochemistry:

This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 mg/mL-0.25 mg/mL with an overnight incubation at 4˚,C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6.0 sodium citrate buffer is recommended.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-33

Target: IL-33 (IL33) (Interleukin 33 (IL33))

Alternative Name: IL-33

Gene ID: 90865

UniProt: O95760

Pathways: Production of Molecular Mediator of Immune Response