Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.
Purification
IQSEC1 antibody is affinity chromatography purified via peptide column.
Immunogen
IQSEC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human IQSEC1.
The immunogen is located within amino acids 60 - 110 of IQSEC1.
IQSEC1
Reactivity: Mouse
WB, IF (cc), IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 647
Application Notes
IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry at 2 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
IQSEC1 antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
IQSEC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1). As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2,3). IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4).