LIMA1 antibody (AA 230-280)

Catalog No. ABIN5690040

Product Details anti-LIMA1 Antibody

Target: LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Binding Specificity: AA 230-280

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist, this antibody will only detect the three largest isoforms.

Purification: LIMA1 antibody is affinity chromatography purified via peptide column.

Immunogen: LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1.

The immunogen is located within amino acids 230 - 280 of LIMA1.

Isotype: IgG

Application Details

Application Notes: LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: LIMA1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: LIMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for LIMA1

Target: LIMA1 (LIM Domain and Actin Binding 1 (LIMA1))

Alternative Name: LIMA1 (LIMA1 Products)

Synonyms: eplin antibody, LIMA1 antibody, EPLIN antibody, MGC146397 antibody, SREBP3 antibody, 1110021C24Rik antibody, 3526402A12Rik antibody, D15Ertd366e antibody, Eplin antibody, LIM domain and actin binding 1 antibody, Lima1 antibody, LIMA1 antibody, lima1 antibody

Background: The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).

Molecular Weight: Predicted: 50, 66, 84 kDa

Observed: 65 kDa

Gene ID: 51474

NCBI Accession: NP_001107018

UniProt: Q9UHB6