SMURF1 antibody (AA 180-230)

Catalog No. ABIN5690258

The Rabbit Polyclonal anti-SMURF1 antibody (ABIN5690258) specifically detects SMURF1 in WB, ELISA and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SMURF1 antibody (AA 180-230) (ABIN5690258)

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMURF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SMURF1 Antibody

Binding Specificity: AA 180-230

Specificity: SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.

Purification: SMURF1 antibody is affinity chromatography purified via peptide column.

Immunogen: SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1.

The immunogen is located within amino acids 180 - 230 of SMURF1.

Isotype: IgG

Application Details

Application Notes: SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SMURF1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: SMURF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for SMURF1

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Alternative Name: SMURF1

Background: SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).

Molecular Weight: Predicted: 80, 83 kDa

Observed: 78 kDa

Gene ID: 57154

NCBI Accession: NP_065162

UniProt: Q9HCE7

Pathways: Regulation of Cell Size