TRIP12 antibody (AA 1930-1980)

Catalog No. ABIN5690304

This Rabbit Polyclonal antibody specifically detects TRIP12 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TRIP12 antibody (AA 1930-1980) (ABIN5690304)

Target: TRIP12 (Thyroid Hormone Receptor Interactor 12 (TRIP12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIP12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRIP12 Antibody

Binding Specificity: AA 1930-1980

Specificity: TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist.

Purification: TRIP12 antibody is affinity chromatography purified via peptide column.

Immunogen: TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12.

The immunogen is located within amino acids 1930 - 1980 of TRIP12.

Isotype: IgG

Application Details

Application Notes: TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TRIP12 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: TRIP12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for TRIP12

Target: TRIP12 (Thyroid Hormone Receptor Interactor 12 (TRIP12))

Alternative Name: TRIP12

Background: Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1). TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3). TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4). TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5).

Molecular Weight: Predicted: 219 kDa

Observed: 220 kDa

Gene ID: 9320

NCBI Accession: NP_004229

UniProt: Q14669

Pathways: Nuclear Hormone Receptor Binding