UVRAG antibody (AA 490-540)

Catalog No. ABIN5690316

This Rabbit Polyclonal antibody specifically detects UVRAG in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for UVRAG antibody (AA 490-540) (ABIN5690316)

Target: UVRAG (UV Radiation Resistance Associated Gene (UVRAG))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UVRAG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UVRAG Antibody

Binding Specificity: AA 490-540

Specificity: UVRAG antibody is human and mouse reactive.

Purification: UVRAG antibody is affinity chromatography purified via peptide column.

Immunogen: UVRAG antibody was raised against an 18 amino acid peptide near the carboxy terminus of human UVRAG.

The immunogen is located within amino acids 490 - 540 of UVRAG.

Isotype: IgG

Application Details

Application Notes: UVRAG antibody can be used for detection of UVRAG by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: UVRAG antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: UVRAG antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for UVRAG

Target: UVRAG (UV Radiation Resistance Associated Gene (UVRAG))

Alternative Name: UVRAG

Background: The class III type phosphoinositide 3-kinase (PI3KC3) / Vps34 regulates vacuolar trafficking as well as autophagy (1). UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (2). UVRAG is highly expressed in brain, lung, kidney and liver and contains one C2 domain, which is involved in calcium-dependent phospholipid binding. Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (3). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (4,5).

Molecular Weight: Predicted: 77 kDa

Observed: 75 kDa

Gene ID: 7405

NCBI Accession: NP_003360

UniProt: Q9P2Y5