IL-15 antibody

Catalog No. ABIN5690436

This Rabbit Polyclonal antibody specifically detects IL-15 in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for IL-15 antibody (ABIN5690436)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-15 Antibody

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-15. Murine IL-15 specific antibody was purified by affinity chromatography employing immobilized mIL-15 matrix.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-15 (5.0 ng/mL), a concentration of 0.4 - 0.5 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect mIL-15 by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15.

Sandwich
To detect mIL-15 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-15 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15.

Western Blot:
To detect mIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-15 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-15

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Alternative Name: IL-15

Background: IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97 % and 73 % amino acid sequence identity with simian and mouse IL15, respectively.

Gene ID: 16168

UniProt: P48346

Pathways: JAK-STAT Signaling, Glycosaminoglycan Metabolic Process