MIA antibody (Biotin)

Catalog No. ABIN5690576

This anti-MIA antibody is a Rabbit Polyclonal antibody detecting MIA in ELISA and WB. Suitable for Human.

Quick Overview for MIA antibody (Biotin) (ABIN5690576)

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIA antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Product Details anti-MIA Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIA. Human MIA specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hMIA by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA.

Sandwich
To detect hMIA by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human MIA as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA.

Western Blot:
To detect hMIA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MIA antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Alternative Name: MIA

Gene ID: 8190

UniProt: Q16674